美國生物homework代寫 單克隆抗體

最初,單克隆抗體被認為是一種生物學工具,在病理診斷和實驗室研究中具有重要的應用價值。由于結合的特異性,它們被用于識別血細胞、其他組織的表型以及其他診斷/成像技術,如免疫組織化學、流式細胞術等。(Weiner 2015)早期的研究表明,單克隆抗體(mAbs)可以通過雜交瘤技術(一種獲得諾貝爾和平獎的技術)輕松而有效地生產出來,使它們可以應用到前面提到的研究中。30年前,單抗還被認為是一種可能的癌癥治療方法。起初,小鼠單克隆抗體被作為一種癌癥治療的試驗,研究結果令人失望。小鼠單克隆抗體,來源于小鼠,特別是實驗室小鼠。由于單克隆抗體與人類免疫系統的相容性較差,半衰期較短,因此給人注射單克隆抗體時出現了問題。具體來說,單克隆抗體招募宿主細胞效應功能的能力較差,對腫瘤部位的穿透能力也較差。然而,當小鼠單克隆抗體能夠發揮這些功能時,卻發現其對腫瘤細胞產生的細胞毒性效果較差,并且產生的復合物能刺激最小的過敏反應,從而完全消除過敏性休克。這種不良的相互作用和過敏反應被稱為人類抗鼠抗體(HAMA)反應。盡管有這些挫折,信息表明單抗治療是可能的。

美國生物homework代寫 單克隆抗體

Initially, mAbs were recognized as biological tools and were essential for applications in pathological diagnosis and laboratory investigation. Due to specificity in binding, they were used to identify the phenotype of blood cells, and other tissues as well as other diagnostic/imaging techniques, such as immunohistochemistry, flow cytometry and various other. (Weiner 2015) Early research showed that monoclonal antibodies (mAbs) could be easily and efficiently produced through hybridoma technology (a technology that won the Nobel peace prize), allowing them to be applied to the research mentioned earlier. Only 30 years ago were mAb’s proposed as a possible therapeutic for cancer. Initially, murine mAbs were trialled as a cancer treatment, and the results from the study were disappointing. Murine mAbs, are derived from mice, specifically laboratory mice. This led to troubles when administering murine mAbs to humans, due to the suboptimal compatibility of the mAb with the human immune system and low half-life. Specifically, the mAbs had a poor ability to recruit host cell effector functions, as well as poor penetration in tumour sites. Though when able to access those functions it was found that murine mAbs were poor at producing a cytotoxic effect on tumour cells, and generation of complexes that stimulated minimal allergic reactions to full out the anaphylactic shock. This poor interaction and an allergic reaction are known as the human anti-murine antibody (HAMA) response. Despite the setbacks, information was elucidated that showed mAb therapy is possible.

本段內容來自網絡 并不是我們的寫手作品 請勿直接剽竊,查重100%,造成后果與本站無關。如需定制論文請記得聯系我們。